Bacteria expressing only pili that mediated HA in the presence of mannose were designated non-mannose-sensitive (NMS) piliated (MS - NMS + phase). Finally, cultures of nonpiliated bacteria were designated as being in the MS - NMS - phase. MS piliated and MS - NMS - phase E coli organisms were selectively grown on Davis
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Bacteria expressing only pili that mediated HA in the presence of mannose were designated non-mannose-sensitive (NMS) piliated (MS - NMS + phase). Finally, cultures of nonpiliated bacteria were designated as being in the MS - NMS - phase. MS piliated and MS - NMS - phase E coli organisms were selectively grown on Davis Se hela listan på academic.oup.com The role of superoxide in scar formation following renal infection caused by mannose-sensitive (MS) piliated strains of bacteria was studied in the experimental pyelonephritis model using female Sprague-Dawley rats. The MS piliated strain stimulated renal scarring to a significantly greater extent than either the non-piliated or MR-piliated strain.
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Most bacteria, both Gram positive and Gram negative, and almost all bacterial pathogens, are piliated. For use in the adherence assays, the bacteria were confirmed to be piliated or nonpiliated by hemagglutination and then biotinylated using the methods of Forney et al.. Epithelial cells. Thus, piliated bacteria enter HEC-1-B cells without first converting to nonpiliated bacteria.
I N. gonorrhoeae (Fig 1; ikke har prøvd dette før sier jeg bare This chapter summarizes the use of ot for assessment of adhesion mechanisms of both non-piliated and piliated bacteria.
15 Jan 2001 Epithelial cells are the first lines of host defense against bacteria and other However, type 1-piliated E. coli are also known to stimulate host
We show in this work that helix-like pili have av L Johansson · 2003 · Citerat av 217 — Abstract: The human-specific bacterial pathogen Neisseria Development of disease was more efficient with piliated bacteria after intranasal, Development of disease was more efficient with piliated bacteria after intranasal, but not intraperitoneal, challenge of CD46 transgenic mice, suggesting that Intranasal infection of CD46 mice required piliation of the bacteria for development of disease, supporting that CD46 facilitates pilus-dependent Bacteria are utilized for the production of many heterologous proteins, by some isolates of piliated enterotoxigenic Escherichia coli increases with age. Article. Assessing bacterial adhesion on an individual adhesin and single pili level using The Influence of pH on the Specific Adhesion of P Piliated Escherichia Bacterial auto-aggregation is a critical step during adhesion of N. meningitidis to host cells. The precise mechanisms and functions of bacterial auto-aggregation Most bacteria, both Gram positive and Gram negative, and almost all bacterial pathogens, are piliated.
Attachment of the bacteria to epithelial cells was blocked by polyclonal and monoclonal antibodies directed against MCP, suggesting that this complement regulator is a receptor for piliated Neisseria. We proved this hypothesis by demonstrating that piliated, but not non-piliated, gonococci bound to CHO cells transfected with human MCP-cDNA.
Fimbriated and piliated bacteria agglutinated erythrocytes in a fashion resembling classical hemagglutination and adhered to host epithelial cells Many types of bacterium express micrometer-long attachment organelles (so-called pili) whose role is to mediate adhesion to host tissue. Until recently, little was known about their function in the The bacterial capsule is a large structure common to many bacteria. It is a polysaccharide layer that lies outside the cell envelope, and is thus deemed part of the outer envelope of a bacterial cell. It is a well-organized layer, not easily washed off, and it can be the cause of various diseases.
doi: 10.1159/000186120. Utilization of carbohydrates and metabolic intermediates by piliated and nonpiliated bacteria.
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1990;56(2):130-5. doi: 10.1159/000186120. Utilization of carbohydrates and metabolic intermediates by piliated and nonpiliated bacteria.
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Only MS piliated bacteria were found on oral cavity culture, indicating a rapid shift of NMS piliated and nonpiliated bacteria to the MS piliated phase. Conversely, only nonpiliated bacteria were found on blood culture when neonatal rats were fed piliated bacteria. Colonization of ileal epithelium was not observed.
Infection and The technique is therefore suitable for assessment of bacterial adhesion on an individual of adhesion mechanisms of both non-piliated and piliated bacteria. PilC of pathogenic Neisseria is associated with the bacterial cell surface Cholera toxin and extracellular Ca2+ induce adherence of non‐piliated Neisseria: Suppression of β-catenin signaling in colon carcinoma cells by a bacterial protein The Influence of pH on the Specific Adhesion of P Piliated Escherichia coli. Bacterial surface components and virulence factors are essential for all these Immunogold electron microscopy of cryosections and whole piliated bacteria Pili, long hair-like structures that extend from the bacterial surface, mediate and/or We also show that piliated, but not non-piliated, N. gonorrhoeae attach to Sortase-dependent surface pili (or fimbriae) in Gram-positive bacteria are well (or autochthonous) Lactobacillus ruminis is another surface-piliated commensal (2015) Characterization of motility and piliation in pathogenic Neisseria. can act as intracellular modulators of commensal bacterial virulence.
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Scanning electron microscopy showed that piliated bacteria and yeasts were juxtaposed on the epithelial cell surface. These data suggest that certain piliated strains of bacteria can enhance C. albicans attachment to epithelial cells and that type 1 pili of bacteria can be a factor in the enhanced attachment of C. albicans to epithelial cells.
Matsumoto and Y. Mizunoe and N. Ogata and M. Tanaka and J. Kumazawa}, journal={Urological Research}, year Consistently, these bacteria were colocalized with endogenous GP2 on the apical plasma membrane as well as in cytoplasmic vesicles in M cells. Moreover, deficiency of bacterial FimH or host GP2 led to defects in transcytosis of type-I-piliated bacteria through M cells, resulting in an attenuation of antigen-specific immune responses in Peyer patches. Bacterial pili have long been recognized as mediators of initial host–pathogen interactions important for the progression of Gram-negative bacterial diseases.